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rabbit anti aggrecan  (R&D Systems)


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    Structured Review

    R&D Systems rabbit anti aggrecan
    Rabbit Anti Aggrecan, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/neurocan/Mouse%2FRat+Neurocan+Antibody/pm41707919-72-9-18
    Average 94 stars, based on 29 article reviews
    rabbit anti aggrecan - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    other:

    Article Title: ECM modulated early kidney development in embryonic organ culture.
    Article Snippet: The use of exogenous signals is gaining importance in renal regenerative therapies.. We wanted to explore the role of extracellular matrix (ECM) constituents on renal structure formation during renal organogenesis.. We used a recently established organ culture setup to expose embryonic kidney rudiments directly to a large set of surface-immobilized or dissolved ECM molecules and growth factors.

    Incubation:

    Article Title: Dysregulation of astrocyte extracellular signaling in Costello syndrome
    Article Snippet: For Western blotting, cells or mouse cortical tissue was lysed in CelLytic M (Sigma-Aldrich) and protease inhibitors (Roche), boiled in SDS–polyacrylamide gel electrophoresis loading buffer plus β-mercaptoethanol for 5 min, and loaded at 30 μg per lane for human cells or 10 μg for brain lysate. .. Membranes were incubated with the following antibodies; p44/42 MAPK (pERK) (Cell Signaling Technology, 9102), GAPDH (Sigma-Aldrich, G8795), GFAP (Chemicon, MAB360), neurocan (R&D Systems, AF5800), and brevican (BD Biosciences, 610894). .. The H-Ras Activation Assay Kit (Cell Biolabs Inc.), Periostin Elisa Kit (Boster, EK0985), and Senescence β-Galactosidase Staining Kit (Cell Signaling Technologies, 9860) were used according to the manufacturers’ instructions.

    Article Title: Neurocan Contributes to Perineuronal Net Development.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Recombinant:

    Article Title: Protofibrillar and Fibrillar Amyloid-β Binding Proteins in Cerebrospinal Fluid
    Article Snippet: .. 196 Recombinant human proteins, agrin (cat. 6624- 197 AG-050), dickkopf-related protein 3 (cat. 1118- 198 DK-050), neurocan (cat. 6508-NC-050), osteopontin 199 (cat. 1433-OP-CF) and SPARC-like protein (cat. 200 2728-SL-050), were purchased from R&D Systems, 201 USA. ..

    Article Title: Borrelia burgdorferi BbHtrA degrades host ECM proteins and stimulates release of inflammatory cytokines in vitro.
    Article Snippet: This article is a U.S. Government work and is in the public domain in the USA., Molecular Microbiology, 90, 241–251 separated in precast Tris-HCl gels (Bio-Rad). .. Commercial reagents: recombinant aggrecan, R&D Systems 1220-PG; biglycan, Sigma B-8041; brevican, R&D Systems 4009-BC; chondroitinase ABC, Sigma C2905; collagen, Millipore CC052; decorin, Sigma D-8428; E-Cadherin, R&D Systems 648-EC; fibronectin, BD Biosciences 354008; neurocan, R&D Systems 6508-NC; and versican, Abnova H00001462-P01. .. Mesenchymal stem cell culture A total of 1 × 106 human mesenchymal stem cells (passage one, Lonza, catalogue #PT-2501) were plated in a T150 flask (Corning) and cultured in growth medium: mesenchymal stem cell basal medium (MSCBM, Lonza, #PT-3238), mesenchymal growth supplements (SingleQuots, Lonza, #PT4105), and 5 ng ml−1 recombinant human fetal growth factor (Shenandoah Biotechnology).

    Blocking Assay:

    Article Title: Neurocan Contributes to Perineuronal Net Development.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Immunohistochemistry:

    Article Title: Neurocan Contributes to Perineuronal Net Development.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.



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    R&D Systems recombinant neurocan ncan
    (A) Experimental scheme. CSPG-rich Matrigel corresponds to 60% <t>Matrigel/Ncan/L15</t> medium. (B–D) Representative super-resolution time-lapse images of ventricular–subventricular zone (V-SVZ)-derived cultured migrating neurons expressing Venus-CAAX (green) and DsRed (red), which label membranes and cytosol, respectively. While “minute-interval” imaging reveals the overall dynamics of the leading process growth cone (B), “second-interval” imaging visualizes the dynamics of fine cellular structures such as lamellipodia and filopodia. (C, D) Yellow, light-blue, and magenta arrows indicate formed, buried, and retracted filopodium, respectively. The structure and dynamics of lamellipodial and filopodial structures should be additionally analyzed by using actin probes such as GFP-actin or EGFP-UtrCH. Numbers indicate seconds from the first imaging frame (B–D). Scale bars, 5 μm (B–D). CSPG, chondroitin sulfate proteoglycan.
    Recombinant Neurocan Ncan, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    (A) Experimental scheme. CSPG-rich Matrigel corresponds to 60% Matrigel/Ncan/L15 medium. (B–D) Representative super-resolution time-lapse images of ventricular–subventricular zone (V-SVZ)-derived cultured migrating neurons expressing Venus-CAAX (green) and DsRed (red), which label membranes and cytosol, respectively. While “minute-interval” imaging reveals the overall dynamics of the leading process growth cone (B), “second-interval” imaging visualizes the dynamics of fine cellular structures such as lamellipodia and filopodia. (C, D) Yellow, light-blue, and magenta arrows indicate formed, buried, and retracted filopodium, respectively. The structure and dynamics of lamellipodial and filopodial structures should be additionally analyzed by using actin probes such as GFP-actin or EGFP-UtrCH. Numbers indicate seconds from the first imaging frame (B–D). Scale bars, 5 μm (B–D). CSPG, chondroitin sulfate proteoglycan.

    Journal: Bio-protocol

    Article Title: Time-Lapse Super-Resolution Imaging and Optical Manipulation of Growth Cones in Elongating Axons and Migrating Neurons

    doi: 10.21769/BioProtoc.5251

    Figure Lengend Snippet: (A) Experimental scheme. CSPG-rich Matrigel corresponds to 60% Matrigel/Ncan/L15 medium. (B–D) Representative super-resolution time-lapse images of ventricular–subventricular zone (V-SVZ)-derived cultured migrating neurons expressing Venus-CAAX (green) and DsRed (red), which label membranes and cytosol, respectively. While “minute-interval” imaging reveals the overall dynamics of the leading process growth cone (B), “second-interval” imaging visualizes the dynamics of fine cellular structures such as lamellipodia and filopodia. (C, D) Yellow, light-blue, and magenta arrows indicate formed, buried, and retracted filopodium, respectively. The structure and dynamics of lamellipodial and filopodial structures should be additionally analyzed by using actin probes such as GFP-actin or EGFP-UtrCH. Numbers indicate seconds from the first imaging frame (B–D). Scale bars, 5 μm (B–D). CSPG, chondroitin sulfate proteoglycan.

    Article Snippet: Recombinant Neurocan (Ncan) (R&D Systems, catalog number: 5800-NC-050) 28.

    Techniques: Derivative Assay, Cell Culture, Expressing, Imaging